abstract：:The nature of postsynaptic sites involved in the uptake and metabolism of striatal 3,4-dihydroxyphenylethylamine (dopamine, DA) was investigated. The accumulation of [3H]DA (10(-7) M) into slices of rat striatum was found to be greatly dependent (greater than 99%) on the presence of sodium ion in the incubation medium...

journal_title：Journal of neurochemistry

authors： Schoepp DD,Azzaro AJ

更新日期：1985-06-01 00:00:00

abstract：:Recent studies have shown that mast cells (MCs) are present in rat brain, that they have a predominantly thalamic localization, and that they contain histamine (HA). However, the degree to which these cells contribute to brain HA levels has remained unclear. Our recent studies of the precise distribution of rat brain ...

journal_title：Journal of neurochemistry

authors： Goldschmidt RC,Hough LB,Glick SD

更新日期：1985-06-01 00:00:00

abstract：:The separation of 38 hypophyseal or hypothalamic peptides and proteins by reversed-phase HPLC is described. The efficacy of several supports is discussed: large-pore reversed-phase silica supports turned out to be most effective in the separation of neuropeptides and proteins. The use of high sodium phosphate and phos...

journal_title：Journal of neurochemistry

authors： Richter WO,Schwandt P

更新日期：1985-06-01 00:00:00

abstract：:The neurochemical profile of a new compound, Lu 19-005 [(+/-)trans-3-(3,4-dichlorophenyl)-N-methyl-1-indanamine hydrochloride], has been investigated. Lu 19-005 is a potent inhibitor of the synaptosomal uptake of 3,4-dihydroxyphenylethylamine (dopamine, DA), noradrenaline (NA), and 5-hydroxytryptamine (5-HT, serotonin...

journal_title：Journal of neurochemistry

authors： Hyttel J,Larsen JJ

更新日期：1985-05-01 00:00:00

abstract：:The objective of the present study was to assess metabolic changes in the neocortex and hippocampus of well-oxygenated or moderately hypoxic rats in which fluorothyl-induced seizures were sustained for 5 or 20 min, or which were allowed recovery periods of 5, 15, or 45 min following cessation of 20-min seizure activit...

journal_title：Journal of neurochemistry

authors： Folbergrová J,Ingvar M,Nevander G,Siesjö BK

更新日期：1985-05-01 00:00:00

abstract：:Previous work from our laboratory points to plasma free tryptophan being a useful predictor of brain tryptophan concentration in many circumstances. Other work, in particular various studies on the acute effects of food intake, has emphasized the roles of plasma total tryptophan and of plasma large neutral amino acids...

journal_title：Journal of neurochemistry

authors： Sarna GS,Kantamaneni BD,Curzon G

更新日期：1985-05-01 00:00:00

abstract：:In rats rendered hyperthyroid by chronic treatment with L-triiodothyronine (T3) hormone there was a 21 and 27% decrease, respectively, in the number of binding sites for [3H]flunitrazepam ([3H]FNZ) and [3H]ethyl-beta-carboline-3-carboxylate ([3H]beta-CCE) without changes in affinity for the two ligands. Two weeks afte...

journal_title：Journal of neurochemistry

authors： Medina JH,De Robertis E

更新日期：1985-05-01 00:00:00

abstract：:Metabolic alterations in amino acids, high-energy phosphates, and intracellular pH during and after insulin hypoglycemia in the rat brain was studied in vivo by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Sequential accumulations of 1H and 31P spectra were obtained from a double-tuned surface coil positi...

journal_title：Journal of neurochemistry

authors： Behar KL,den Hollander JA,Petroff OA,Hetherington HP,Prichard JW,Shulman RG

更新日期：1985-04-01 00:00:00

abstract：:Angiotensin-converting enzyme (ACE) activity was measured in microvessels prepared from cerebral cortices of 4-week-old spontaneously hypertensive rats (SHR). The Vmax value of the ACE activity in the cerebral microvessels of SHR was lower than that of Wistar Kyoto controls of the same age by 25% without difference in...

journal_title：Journal of neurochemistry

authors： Kobayashi H,Wada A,Izumi F,Take K,Magnoni MS

更新日期：1985-04-01 00:00:00

abstract：:An enzyme-linked immunoadsorbent assay (ELISA) for neurofilament protein was utilised to quantify the effect of exogenous ganglioside on neurite regeneration in cultures of dorsal root ganglion neurones. In contrast to nerve growth factor (NGF), ganglioside GM1 (100 micrograms/ml) failed to support neuronal survival a...

journal_title：Journal of neurochemistry

authors： Doherty P,Dickson JG,Flanigan TP,Walsh FS

更新日期：1985-04-01 00:00:00

abstract：:The contents of cystathionine and taurine, as well as cystathionine beta-synthase activity in various regions of the brains of normal and DL-propargylglycine-treated rats, were measured. The content of cystathionine in each region of brain increased gradually from 0.5 mg to 20 mg/200 g body weight in relation to the d...

journal_title：Journal of neurochemistry

authors： Kodama H,Ikeda H,Awata S,Nakayama K

更新日期：1985-04-01 00:00:00

abstract：:Animals treated with high doses of amphetamines have been used as a model of schizophrenia due to the similarities between the psychosis associated with this mental disorder and that induced by chronic amphetamine abuse. When administered to naive rats in high doses, the amphetamine-like CNS stimulant methamphetamine ...

journal_title：Journal of neurochemistry

authors： Schmidt CJ,Sonsalla PK,Hanson GR,Peat MA,Gibb JW

更新日期：1985-03-01 00:00:00

abstract：:We have observed recently that in vitro lipoperoxidation is enhanced in Down's syndrome brain homogenates of prenatal age. As this may relate to the composition of polyunsaturated acyl groups (PUFA) in phospholipids, we have examined the PUFA of ethanolamine and serine phosphoglycerides (EPG and SPG), which are partic...

journal_title：Journal of neurochemistry

authors： Brooksbank BW,Martinez M,Balazs R

更新日期：1985-03-01 00:00:00

abstract：:The binding characteristics of [3H] alpha-dihydropicrotoxinin to the picrotoxinin binding site were investigated in membrane preparations of adult rat forebrain and living cultures of rat cerebral cortex. The binding of [3H]alpha-dihydropicrotoxinin to rat forebrain was decreased by lysing, treating with Triton X-100,...

journal_title：Journal of neurochemistry

authors： White WF,Snodgrass SR,Dichter MA

更新日期：1985-03-01 00:00:00

abstract：:The inhibition of [3H]5-hydroxytryptamine [( 3H]5-HT) binding in rat brain by 1-[2-(3-bromoacetamidophenyl)ethyl]-4-(3-trifluoromethylphenyl) piperazine (BrAcTFMPP) and that by spiperone were compared. Spiperone inhibition of [3H]5-HT binding in cortex was consistent with displacement from two sites with dissociation ...

journal_title：Journal of neurochemistry

authors： Ransom RW,Asarch KB,Shih JC

更新日期：1985-03-01 00:00:00

abstract：:The function of membrane phospholipids (PL) in the regulation of gamma-aminobutyric acid (GABA) transport and GABA carrier binding has been investigated in organized cultures of rat cerebral cortex. The cellular lipid composition has been changed by growing the cells in a delipidated nutrient solution or by short-term...

journal_title：Journal of neurochemistry

authors： Giesing M,Gerken U,Kastrup H

更新日期：1985-03-01 00:00:00

abstract：:The effects of gamma-aminobutyric acid (GABA) on the extracellular levels of taurine and on excitability in the dentate gyrus were studied in anesthetized rats by the dialytrode technique. The dentate gyrus was perfused by means of a dialytrode with Krebs-Ringer-bicarbonate or GABA solutions. Amino acid contents in pe...

journal_title：Journal of neurochemistry

authors： Lerma J,Herranz AS,Herreras O,Muñoz D,Solís JM,del Río RM,Delgado JM

更新日期：1985-03-01 00:00:00

abstract：:The activation by endogenous dopamine of the inhibitory 3,4-dihydroxyphenylethylamine (dopamine) receptors modulating the electrically evoked release of [3H]acetylcholine [( 3H]ACh) and [3H]dopamine in rat striatal slices is a function of the concentration of dopamine accumulated in the synaptic cleft during electrica...

journal_title：Journal of neurochemistry

authors： Baud P,Arbilla S,Langer SZ

更新日期：1985-02-01 00:00:00

abstract：:Cultures of myelinated SJL/J fetal mouse spinal cord were incubated with serum and lymphoid cells from syngeneic animals with experimental allergic encephalomyelitis (EAE) induced by syngeneic spinal cord homogenate (SSCH) in complete Freund's adjuvant or others injected with complete Freund's adjuvant alone. After 24...

journal_title：Journal of neurochemistry

authors： Roth GA,Bornstein MB,Röyttä M,Lyman WD,Raine CS

更新日期：1985-02-01 00:00:00

abstract：:The time course of the reduction in brain protein synthesis following transient bilateral ischemia in the gerbil was characterized and compared with changes in a number of metabolites related to brain energy metabolism. The recovery of brain protein synthesis was similar following ischemic periods of 5, 10, or 20 min;...

journal_title：Journal of neurochemistry

authors： Nowak TS Jr,Fried RL,Lust WD,Passonneau JV

更新日期：1985-02-01 00:00:00

abstract：:We examined the effects of treatments affecting norepinephrine release on the number of norepinephrine reuptake recognition sites as reflected by desipramine binding. To do this, we used manipulations having similar presynaptic but contrasting postsynaptic effects. Presynaptic inhibition by 6-hydroxydopamine lesion or...

journal_title：Journal of neurochemistry

authors： Swann AC,Duman R,Hewitt L

更新日期：1985-02-01 00:00:00

abstract：:Saturable and specific binding sites for 5-[3H]hydroxytryptamine (5-HT, serotonin) characterized by a KD of 3.5-4.5 nM were detected in the chick embryo brain and were shown to develop linearly as a function of age, weight, and protein content. Saturation and displacement studies using unlabeled 5-HT as the displacing...

journal_title：Journal of neurochemistry

authors： Soblosky JS,Jeng I

更新日期：1985-02-01 00:00:00

abstract：:Serotonin N-acetyltransferase, an enzyme of the pineal gland, converts serotonin to N-acetylserotonin. The activity of this enzyme is induced by norepinephrine in the evening to reach high levels during the dark phase. Delta-sleep-inducing peptide, a humoral sleep factor, also seems to affect circadian rhythms. Intrav...

journal_title：Journal of neurochemistry

authors： Graf MV,Kastin AJ,Schoenenberger GA

更新日期：1985-02-01 00:00:00

abstract：:Kinetic studies on the action of monoamine oxidase (MAO) in the regulation of Na+,K+-ATPase were performed using 3-methoxy-4-hydroxybenzaldehyde (MHB), which is an analogue of 3-methoxy-4-hydroxy-phenylacetylaldehyde (product of MAO-catalysed reaction with dopamine as substrate). It was observed that at 2.6 microM MHB...

journal_title：Journal of neurochemistry

authors： Mayanil CS,Baquer NZ

更新日期：1985-01-01 00:00:00

abstract：:Acetylcholinesterase (AChE; EC 3.1.1.7) extracted in 1% Triton X-100 from rabbit brain was purified 2,000-fold by chromatography on agarose conjugated with a monoclonal antibody directed against human red blood cell cholinesterase. After elution from the immunoadsorbent with pH 11 buffer, the preparation was purified ...

journal_title：Journal of neurochemistry

authors： Mintz KP,Brimijoin S

更新日期：1985-01-01 00:00:00

abstract：:The subcellular distribution of peptide histidine isoleucine amide (PHI)-27-like peptides (PLP) was investigated in rat cerebral cortex and whole rat brain in comparison with the distribution of vasoactive intestinal peptide (VIP). The highest content of PLP was found in the crude mitochondrial fraction (P2) and was a...

journal_title：Journal of neurochemistry

authors： Korchak DM,Gysling K,Beinfeld MC

更新日期：1985-01-01 00:00:00

abstract：:Voltage-dependent calcium channels from ileal smooth muscle can be affinity-labeled with a [3H]dihydropyridine isothiocyanate radioligand. We examined the binding of this agent to brain membranes, to compare the properties of calcium channel drug binding sites in brain with those previously described in ileum. In brai...

journal_title：Journal of neurochemistry

authors： Greenberg DA,Cooper EC,Carpenter CL

更新日期：1985-01-01 00:00:00

abstract：:1-Methyl-4-phenyl-1,2,5,6-tetrahydropyridine (MPTP) is known to cause a destruction of the dopaminergic nigrostriatal pathway in certain animal species including mice. MPTP and some structurally related analogs were tested in vitro for their capacity to inhibit the uptake of [3H]3,4-dihydroxyphenylethylamine-([3H]DA),...

journal_title：Journal of neurochemistry

authors： Heikkila RE,Youngster SK,Manzino L,Cabbat FS,Duvoisin RC

更新日期：1985-01-01 00:00:00

abstract：:To assess maturation of central serotonin and catecholamine pathways at birth, we measured lumbar CSF homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), stable acid metabolites of dopamine and serotonin, using HPLC with electrochemical detection. CSFs from 57 neonates (38 premature and 19 at term) and 13...

journal_title：Journal of neurochemistry

authors： Silverstein FS,Donn S,Buchanan K,Johnston MV

更新日期：1984-12-01 00:00:00

abstract：:Previous studies have purified from brain a Ca2+/calmodulin-dependent protein kinase II (designated CaM-kinase II) that phosphorylates synapsin I, a synaptic vesicle-associated phosphoprotein. CaM-kinase II is composed of a major Mr 50K polypeptide and a minor Mr 60K polypeptide; both bind calmodulin and are phosphory...

journal_title：Journal of neurochemistry

authors： Shields SM,Vernon PJ,Kelly PT

更新日期：1984-12-01 00:00:00

abstract：:A clone of murine neuroblastoma (N1E-115) was shown to have functional receptors for the nonapeptide bradykinin. These receptors mediated a large, rapid (about 1 min to peak) and calcium-dependent increase in cyclic GMP. The median effective concentration (EC50) averaged 1.4 nM. In addition, this event was inhibited b...

journal_title：Journal of neurochemistry

authors： Snider RM,Richelson E

更新日期：1984-12-01 00:00:00

abstract：:Both vasoactive intestinal peptide (VIP) and glucagon rapidly elevated cyclic AMP levels in embryonic chick retinal pigment epithelium (RPE), in culture as well as in freshly dissected tissue. In cultured cells, the half-maximal activities of VIP and glucagon were 5 X 10(-8) M and 3 X 10(-8) M, respectively. After 3 m...

journal_title：Journal of neurochemistry

authors： Koh SW,Chader GJ

更新日期：1984-12-01 00:00:00

abstract：:We have found NAD to be rapidly degraded by extracellular enzymes present on intact rat brain synaptosomes. The enzyme involved had the specificity of an NADase cleaving the molecule at the nicotinamide-glycoside linkage and was inhibited by nicotinamide mononucleotide (NMN). This inhibitor did not displace specific b...

journal_title：Journal of neurochemistry

authors： Snell CR,Snell PH,Richards CD

更新日期：1984-12-01 00:00:00

abstract：:1-Piperideine, 5-aminopentanoic acid, and its lactam, 2-piperidone, were identified as metabolites of cadaverine in 10,000 g mouse liver supernatants to which diamine oxidase had been added. Both metabolites were also found when the cadaverine metabolite 1-piperideine was incubated with the preparation which suggested...

journal_title：Journal of neurochemistry

authors： Callery PS,Geelhaar LA

更新日期：1984-12-01 00:00:00

abstract：:Specific D2 binding in rat striatum was characterized and then the effects of chronic disruption of dopaminergic activity on antagonist and agonist binding to these sites were studied. D2 receptors were defined as those sites capable of binding [3H]spiperone in the presence of cinanserin, a 5-HT2 antagonist, but not i...

journal_title：Journal of neurochemistry

authors： MacKenzie RG,Zigmond MJ

更新日期：1984-11-01 00:00:00

abstract：:Intact neurofilaments (NF) purified from mammalian brain and spinal cord promote the assembly of microtubules in solutions of pure phosphocellulose (PC)-purified tubulin. This assembly is temperature-dependent and is inhibited by mitotic spindle inhibitors. The ability of NF to induce microtubule formation is 20% of t...

journal_title：Journal of neurochemistry

authors： Leterrier JF,Wong J,Liem RK,Shelanski ML

更新日期：1984-11-01 00:00:00

abstract：:The effect of the glutamine synthetase (GS) inhibitor, methionine sulfoximine (MSO), on glutamate levels in, and glutamate release from, rat striatal tissue was examined. Tissue levels of glutamate were unchanged 24 h after an intraventricular injection of MSO, but tissue glutamine levels were decreased 50%. Calcium-d...

journal_title：Journal of neurochemistry

authors： Rothstein JD,Tabakoff B

更新日期：1984-11-01 00:00:00

abstract：:Single-bilayer vesicles of myelin are desirable for studying myelin development and metabolism. Accordingly, our interest was drawn to a procedure for vesiculating myelin (Steck et al., Biochim, Biophys. Acta 509, 397-408, 1978). We used X-ray diffraction analysis to examine these putative vesicle preparations because...

journal_title：Journal of neurochemistry

authors： Sedzik J,Toews AD,Blaurock AE,Morell P

更新日期：1984-11-01 00:00:00

abstract：:Sprague-Dawley rats were fed one of three purified diets--10% corn oil, 10% hydrogenated coconut oil, or 10% linseed oil--through two generations. At 60-80 days of age the animals were sacrificed. The fatty acyl composition of phosphatidylcholine, phosphatidylethanolamine, plasmalogen phosphatidylethanolamine, and com...

journal_title：Journal of neurochemistry

authors： Brown ML,Marshall LA,Johnston PV

更新日期：1984-11-01 00:00:00

abstract：:Serotonin S2, benzodiazepine, and muscarinic receptors showed different regional distributions in the human brain but were present in all cortical areas. The laminar distributions of [3H]ketanserin, [3H]diazepam, and [3H]quinuclidinylbenzilate were investigated in the temporal cortex and revealed a high density in the...

journal_title：Journal of neurochemistry

authors： Luabeya MK,Maloteaux JM,Laduron PM

更新日期：1984-10-01 00:00:00